The Haematological profiles of high fat diet mice model with moringa oleifera leaves ethanol extract treatment

Abstract

Characteristics of obesity as dysfunction of adipose tissue and chronic low-grade inflammation can have impacts to haematological parameters. The Moringa oleifera leaves have been used empirically to treat metabolic and blood related diseases. However, it is still unknown whether Moringa oleifera leaves can influence haematological parameters in high fat diet. The study aimed to investigate the haematological parameter of high fat diet mice in parallel with Moringa oleifera leaves ethanol extract (MOLE). Forty male DDY mice in 5 weeks of age were randomly divided into five groups as follows: The SD group was fed with a standard diet, the HFD group was fed with high fat diet, the HFD+S group was fed with high fat diet and simvastatin (0,8 mg/20gBW/day). The HFD+MOLE1 and the HFD+MOLE2 groups were fed with high fat diet and MOLE in a dose of 5,6 mg/20gBW/day and 11,2 mg/20gBW/day, respectively. The experiment was performed for 7 weeks. At the end of the experiment, blood was drawn for haematology analysis of blood parameters. We found that sub-chronic high fat diet might alter the haematological parameters. Two different doses of MOLE might have potencies to prevent the worsening caused by those alterations, by increased of haemoglobin[12,9(10,4-15,3) vs 13,9(12,9-18,2) and 14(10,2-14,8), p>0,05)], slight decreased of WBC [(3,59±1,974) vs (3,433±1,747) and (3,42±1,014), p>0,05]. Additionally, the MOLE might have an effect to decreasing the granulocyte percentage [18,5(9,2-43,6) vs 11,5(7,6-18,2) and 8,05(3,2-25,1), p>0,05], and slight decreased of MPV [4,2(2,6-6,7) vs 3,6(3,2-4,1) and 3,55(2,6-6,8), p>0,05]. In summary, the study showed that MOLE might have potencies to prevent the worsening of hematological parameters in the sub-chronic high fat diet.