The extraction and regeneration of resin XAD-16 in the purification of epothilones

Abstract

The XAD-16 resins, which can capture the epothilones in situ and remove them from the culture broth, are always used in isolation and purification of epothilones during the fermentation process. In this paper, the effects of five different organic solvents (methanol, alcohol, acetone, ethyl acetate, carbon tetrachloride) on the epothilone extraction and regeneration of macroporous adsorption resin XAD-16 were surveyed. Of five organic solvents, methanol showed a much better effect on epothilone yield than the others. Also, the differences in structure between the fresh-resin and the regenerative e-resin with methanol by atomic force microscope were analyzed. As the results, pore size of methanol-resin was larger than fresh-resin’s, which lead it more conducive to the adsorption of epothilones and regenerative-resin treated with methanol was sturdier. Result of the impact experiment showed the macroporous adsorption resin XAD-16 could be used for at least five times under methanol treatment.