Mass Production Of Tetraselmis Suecica (Kylin) Butch. (Prasinophyceae), Under Different Concentrations Of Nutrients And Additions Of Carbon Dioxide

Abstract

A series of four experiments with Tetmsehis suecica was carried out under laboratory conditions in order to learn the production yield in volumes of 1,000 litres, with salinity of 28o/oo, with absence and addition of buffer and carbon dioxide, air flow of 25 I/min and average time of addition of carbon dioxide of two and a half minutes, at a pressure of 50 kg/cm2 to keep the pH equal to or below 7.5. A maximum density of 508,000 cells/ml was obtained with a ratio of SO/10 g/l of NaNOs/ NaH2P04, with an average constant of growth of 0.35 and 0.50 doublings per day. With a quarter and with half of the nutrients without buffer, a higher constant of growth was recorded, although it did not differ significantly at 95% confidente in relation to the control experiment. Productivity per unit of culture volume of T. suecica showed that on using low con- centrations of nutrients plus carbon dioxide, good population yields relative to the control are obtained. Productivity was not affected by temperature since it was kept constant at 19 + loC, but it decreases when the supplement of carbon dioxide is insufficient to keep the pH between 7.2-7.5