Antioxidant 1 copper chaperone gene expression and copper levels in dog osteosarcoma patients

Abstract

Twenty-four dogs with OS underwent limb amputation. Serum, OS tumour, and normal bone were harvested at time of surgery. RNA was extracted and gene expression was performed using quantitative polymerase chain reaction (qPCR). Tissue and blood copper concentrations were also determined with spectrophotometry. Compared to bone, tumour samples had significantly higher expressions of antioxidant 1 copper chaperone (ATOX1, p =.0003). OS tumour copper levels were significantly higher than that of serum (p