In most protocols for the Agrobacterium-mediated transformation of wheat, the preferred target tissues are immature embryos. However, transformation methods relying on immature embryos require the growth of plants under controlled conditions to provide a continuous supply of good-quality target tissue. The use of mature embryos as a target tissue has the advantage of only requiring good-quality seed as the starting material. Here we describe a transformation method based on the Agrobacterium-mediated transformation of callus cultures derived from mature wheat embryos of the genotype Bobwhite S56.
CITATION STYLE
Medvecká, E., & Harwood, W. A. (2015). Wheat (Triticum aestivum L.) transformation using mature embryos. Methods in Molecular Biology (Clifton, N.J.), 1223, 199–209. https://doi.org/10.1007/978-1-4939-1695-5_16
Mendeley helps you to discover research relevant for your work.