Acetolactate synthase (ALS) is an enzyme in the biosynthetic pathway for branched-chain amino acids, and bispyribac-sodium (BS), a pyrimidinyl carboxy herbicide, is a well-known inhibitor of ALS activity. However, it appears that a mutated form of rice ALS [OsmALS (W548L/S627I)] confers resistance to BS. We succeeded in using OsmALS with native OsALS promoter and terminator region for a selection marker of rice transformation. Because this selection marker cassette is originally from the rice endogenous genome, it can be expected to be publicly acceptable. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Endo, M., Shimizu, T., & Toki, S. (2012). Selection of transgenic rice plants using a herbicide tolerant form of the acetolactate synthase gene. Methods in Molecular Biology, 847, 59–66. https://doi.org/10.1007/978-1-61779-558-9_6
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