This chapter describes protocols for preparing 15N-labeled proteins (ubiquitin is used as an example) using Escherichia coli cells (with purification) and the wheat germ cell-free system (without purification). A comparison of 1H-15N heteronuclear single-quantum coherence (HSQC) spectra of yeast ubiquitin prepared using each method indicates that this wheat germ cell-free system may be used for rapid nuclear magnetic resonance analyses of proteins without purification.
CITATION STYLE
Kohno, T. (2005). Production of proteins for NMR studies using the wheat germ cell-free system. Methods in Molecular Biology (Clifton, N.J.), 310, 169–185. https://doi.org/10.1007/978-1-59259-948-6_12
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