The patch clamp technique revolutionized the study of ion channels and is considered the gold standard of measuring ion channel activity, from the academic laboratory to industrial-scale drug screening. This technique enables the study of ion channels, from single molecules up to the whole-cell ion channel population, and in their native environment. Whilst the study of single protein molecular behavior is the ultimate goal of biophysicists from all fields, this is a routine ability for ion channel specialists. This chapter is aimed at helping the beginner to design patch clamp experiments and to obtain the fundamental micropipette configurations with mammalian cells: cell-attached patch, whole cell, inside-out patch, and outside-out patch. © 2013 Springer Science+Business Media, LLC.
CITATION STYLE
Lippiat, J. D., & Wrighton, D. C. (2013). Conventional micropipette-based patch clamp techniques. Methods in Molecular Biology, 998, 91–107. https://doi.org/10.1007/978-1-62703-351-0_7
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