CHEMICAL CONSTITUENTS AND ANTI-INFLAMMATORY EFFECTS OF SOME STILBENOIDS FROM DIPTEROCARPUS RETUSUS FRUITS OF VIET NAM

Abstract

Dipterocarpus retusus Blume growing in Northern Viet Nam is a member of the plants family Dipterocarpaceae. Previous studies indicated that various Dipterocarpus species contain oleoresin, triterpenes, phenolics, stilbenoids, and coumarins with interesting biological activities. In a previous paper, we reported the isolation and characterization of five compounds, eleutherol, trans-resveratrol, polydatin, β-sitosterol, and β-sitosterol-3-O-β-D-glucopyranoside from fruits of D. retusus Blume. In continuation of our interest on this plant, this article describes the phytochemistry and anti-inflammatory activities of D. retusus Blume collected in Phu tho province. From fruits of Dipterocarpus retusus Blume (Dipterocarpaceae) growing in Viet Nam, five compounds, including (-)-trans-ε-viniferin (1), paucifloroside A (2), ursolic acid (3), quercetin (4), and catechin (5), were isolated and identified by spectroscopic methods and comparison with literature data. This study also reports the anti-inflammatory effect of methanol and ethyl acetate extracts and stilbenoid compounds via their inhibitory activity against the production of nitric oxide (NO) in RAW 264.7 macrophages cells stimulated by lipopolysaccharide (LPS). All data are presented as means of three replicates ± standard deviations. The test concentrations of 30 and 100 (μg/mL all samples were capable of inhibiting NO production with varying grades of cytotoxicities. The stilbenoids: Polydatin (8), trans-e-viniferin (1) and paucifloroside A (2) showed both high anti-inflammatory activity and low cytotoxicity against the testing cells (IC50 value: Polydatin = 0.46 ± 0.21 (μg/mL, trans-ε-viniferin = 2.51 ± 0.35 (μg/mL and paucifloroside A = 16.60 ± 1.56 (μg/mL).