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Book Chapter

Three-dimensional Imaging on Confocal and Wide-field Microscopes

  • Carrington W
  • Fogarty K
  • Lifschitz L
  • et al.
Springer US, (1990), 151-161
DOI: 10.1007/978-1-4615-7133-9_14
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Abstract

The conventional (wide-field) light microscope accepts light from planes above and below the plane of focus. This lack of depth discrimination is the main limitation of the wide-field microscope for 3D imaging. The confocal microscope rejects this out-of-focus light...

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Carrington, W. A., Fogarty, K. E., Lifschitz, L., & Fay, F. S. (1990). Three-dimensional Imaging on Confocal and Wide-field Microscopes. In Handbook of Biological Confocal Microscopy (pp. 151–161). Springer US. https://doi.org/10.1007/978-1-4615-7133-9_14

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