Evaluation of Antioxidant Activities by use of Various Extracts from Abutilon Pannosum and Grewia Tenax in the Kachchh Region

Abstract

Abutilon pannosum (AP) and Grewia tenax (GT) is a significant medicinal plant widely used in the kachchh region against several diseases. This study was devoted to the determination of antioxidant activity of A. pannosum and G. tenax leaf. Determination of antioxidant activity (AOA) after their successive soxhlet extraction using various solvents with different polarities like n-hexane, benzene, chloroform, acetone, ethyl acetate, acetonitrile and ethanol and also petroleum ether, isopropanol, methanol and water. In the present study following findings were observed: (i) Determination of the AOA in different solvent leaf extracts (DSE) of AP and GT by 2, 2-diphenyl-1-picrylhydrazyl (DPPH), total antioxidant capacity (TAC) by the phosphomolybdenum method (PM) and 2, 2-azinobis-(3-ethylbenzothiazoline-6-sulphonate) (ABTS) method. All these AOA increased with increasing concentrations in a quantity dependent method. The AOA was measured with IC50 values of the extracts ranged between AP: 0.338μg/ml to 1.460μg/ml and GT: 0.023μg/ml to 0.652μg/ml. IC50 values is contrariwise proportional to the AOA of plant extracts. The lowest IC 50 revenues had the maximum antioxidant capacity. The maximum radical scavenging effect was detected in AP (with IC50 = 0.338μg ml/1by methanolic fraction of ethyl acetate) and GT (with IC50 = 0.023μg/ml by the methanolic fraction of acetone). We conclude after the some contact interval there were no significant change could be shown in some types of sample extract. The data obtained in the present study suggests that the different solvent extract and its methanolic fraction of AP and GT leaves have potent antioxidant activity against free radicals, prevent oxidative damage to major biomolecules and afford significant protection against oxidative damage in the liver.