Objectives: There is a need for alternative strategies to combat and prevent antibiotic-resistant bacterial infections. Here, we assessed the potential for bacteriophage prophylaxis in the context of experimental ventilator-associated pneumonia due to methicillin-resistant Staphylococcus aureus in rats. Design: Nebulized phages (aerophages) were delivered to the lungs of rats using a modified vibrating mesh aerosol drug delivery system. Animals were intubated and ventilated for 4 hours, at which point they were infected with methicillin-resistant S. aureus strain AW7 via the endotracheal tube, extubated, and then monitored for 96 hours. Setting: Ventilator-associated pneumonia. Subjects: Male Wistar rats. Interventions: A single application of aerophages prior to ventilation at one of two concentrations (∼1010 plaque forming units/mL or ∼1011 plaque forming units/mL). Measurements and Main Results: 1) Animal survival at 96 hours, 2) enumeration of bacteria and phages in the lungs and spleen, and 3) lung tissue histopathology. Animals that received aerophages prior to ventilation and methicillin-resistant S. aureus challenge showed a higher survival rate compared with untreated controls (60% for animals that received 3 × 1010plaque forming units; 70% for animals that received 3 × 1011plaque forming units; 0% for controls; p < 0.01 for each treatment versus untreated). Surviving animals that received aerophage prophylaxis had fewer methicillin-resistant S. aureus in the lungs compared with untreated control animals that succumbed to pneumonia (1.6 × 106colony forming units/g vs 8.0 × 108; p < 0.01). Conclusions: Prophylactically administered nebulized bacteriophages reduced lung bacterial burdens and improved survival of methicillin-resistant S. aureus infected rats, underscoring its potential in the context of ventilator-associated pneumonia.
CITATION STYLE
Prazak, J., Valente, L., Iten, M., Grandgirard, D., Leib, S. L., Jakob, S. M., … Cameron, D. R. (2020). Nebulized Bacteriophages for Prophylaxis of Experimental Ventilator-Associated Pneumonia Due to Methicillin-Resistant Staphylococcus aureus. Critical Care Medicine, 48(7), 1042–1046. https://doi.org/10.1097/CCM.0000000000004352
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