Polyclonal antibodies consist of a mixture of antibodies produced by multiple B-cell clones that have differentiated into antibody-producing plasma cells in response to an immunogen. Polyclonal antibodies raised against an antigen recognize multiple epitopes on a target molecule, which results in a signal amplification in indirect immunoassays including immune-electron microscopy. In this chapter, we present a basic procedure to generate polyclonal antibodies in rabbits.
CITATION STYLE
Nakazawa, M., Mukumoto, M., & Miyatake, K. (2016). Production and purification of polyclonal antibodies. In Methods in Molecular Biology (Vol. 1474, pp. 49–59). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6352-2_3
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