Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR

20Citations
Citations of this article
32Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

We validated the use of stored samples for Chlamydia trachomatis research. C. trachomatis DNA was detected by real-time PCR in clinical (urine and self-taken vaginal swabs) and spiked samples using six different media, five different time points (up to 2 years), and four different temperature conditions. C. trachomatis was detected in all 423 samples, and no clinically relevant degradation impact was detected. Copyright © 2013, American Society for Microbiology. All Rights Reserved.

Cite

CITATION STYLE

APA

Van Dommelen, L., Wolffs, P. F. G., Van Tiel, F. H., Dukers, N., Herngreen, S. B., Bruggeman, C. A., & Hoebe, C. J. P. A. (2013). Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR. Journal of Clinical Microbiology, 51(3), 990–992. https://doi.org/10.1128/JCM.02631-12

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free