We validated the use of stored samples for Chlamydia trachomatis research. C. trachomatis DNA was detected by real-time PCR in clinical (urine and self-taken vaginal swabs) and spiked samples using six different media, five different time points (up to 2 years), and four different temperature conditions. C. trachomatis was detected in all 423 samples, and no clinically relevant degradation impact was detected. Copyright © 2013, American Society for Microbiology. All Rights Reserved.
CITATION STYLE
Van Dommelen, L., Wolffs, P. F. G., Van Tiel, F. H., Dukers, N., Herngreen, S. B., Bruggeman, C. A., & Hoebe, C. J. P. A. (2013). Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR. Journal of Clinical Microbiology, 51(3), 990–992. https://doi.org/10.1128/JCM.02631-12
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