Abstract
The aim of this chapter is to describe a method used to evaluate gene expression and microRNAs (miRNAs) in bone cells or tissue using Reverse transcription and quantitative Polymerase Chain Reaction (RT-qPCR), and a method to assess chromogenic in situ hybridization (CISH) on Formalin Fixed Paraffin Embedded (FFPE ) mouse bone tissue to detect both DNA and mRNA transcripts using the double digoxigenin (DIG) locked nucleic acid (LNA™) probes.
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Moukengue, B., Amiaud, J., Jacques, C., Charrier, C., Ory, B., & Lamoureux, F. (2019). Analysis of mRNA, miRNA, and DNA in bone cells by RT-qPCR and in situ hybridization. In Methods in Molecular Biology (Vol. 1914, pp. 169–196). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8997-3_9
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