The direct uptake of DNA by naked plant cells (protoplasts) provides an expression system of exception for the quickly growing research in non-model plants, fuelled by the power of next-generation sequencing to identify novel candidate genes. Here, we describe a simple and effective method for isolation and transformation of protoplasts, and illustrate its application to several plant materials.
CITATION STYLE
Duarte, P., Ribeiro, D., Carqueijeiro, I., Bettencourt, S., & Sottomayor, M. (2016). Protoplast transformation as a plant-transferable transient expression system. In Methods in Molecular Biology (Vol. 1405, pp. 137–148). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3393-8_13
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