Development and Validation of HPLC Analytical Method for Chlorantraniliprole in Bulk and in the Formulation

Abstract

BACKGROUND: Chlorantraniliprole is widely used as a pesticide. It is only soluble in dimethyl formamide. However, most of the reported methods used acetonitrile and other solvents. AIM: To develop rapid, accurate, precise, and sensitive HPLC method for chlorantraniliprole. MATERIALS AND METHODS: 10 μG/mL of chlorantraniliprole containing solution was injected into the HPLC system and run in different solvent systems. 10 μG/mL solutions of chlorantraniprole was injected in a column with 20 μL microsyringe. The chromatogram was run for appropriate minutes with mobile phase water. The flow rate was set to 1 mL/min and detection was carried out at wavelength 270 nm. The method is validated by measuring the limit of quantitation, limit of detection, repeatability, intraday precision, inter-day precision, and accuracy. Analysis of marketed formulation, bringle, and tomato for chlorantraniprole content was also made for the developed analytical method. RESULTS: There was linearity of chlorantraniliprole for calibration curve in between 1 and 5 μG/mL concentration. The perfect sharp peak observed in water at a retention time of 6.28 min. The limit of detection and the limit of quantitation were 0.0050 μG/mL and 0.0152 μG/mL, respectively. The study reported 99.55% repeatability, 99.49% intraday precision, 99.65% interday precision, and 99.27% accuracy. CONCLUSION: The rapid, accurate, precise, and sensitive HPLC method for the detection of chlorantraniliprole using dimethyl formamide was developed.