Improved microscopy with ultraviolet surface excitation (MUSE) using high-index immersion illumination

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Abstract

We characterize a high-index immersion illumination dipping cap providing improved resolution by refracting light at the tissue interface. We show improved resolution and contrast with deconvolution and focal stacking for high-throughput microscopy.

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Ching-Roa, V., Huang, C., & Giacomelli, M. (2021). Improved microscopy with ultraviolet surface excitation (MUSE) using high-index immersion illumination. In Optics InfoBase Conference Papers. Optica Publishing Group (formerly OSA). https://doi.org/10.1364/boe.435520

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