MYEOV (myeloma overexpressed gene) drives colon cancer cell migration and is regulated by PGE2

Abstract

Introduction: We have previously reported that Myeov (MYEloma OVerexpressed gene) expression is enhanced in colorectal cancer (CRC) and that it promotes CRC cell proliferation and invasion. The role of Myeov in CRC migration is unclear. ProstaglandinE2 (PGE 2) is a known factor in promoting CRC carcinogenesis. The role of PGE 2in modulating Myeov expression has also not been defined. Aim: To assess the role of Myeov expression in CRC cell migration and to evaluate the role of PGE 2in Myeov bioactivity. Methods: siRNA mediated Myeov knockdown was achieved in T84 CRC cells. Knockdown was assessed using quantitative real time PCR. The effect of knockdown on CRC cell migration was assessed using a scratch wound healing assay. Separately, T84 cells were treated with PGE 2(0.00025 μ M, 0.1 μ M and 1μ M) from 30 min to 3 hours and the effect on Myeov gene expression was assessed using real time PCR. Results: Myeov knockdown resulted in a significant reduction in CRC cell migration, observable as early as 12 hours (P < 0.05) with a 39% reduction compared to control at 36 hours (p < 0.01). Myeov expression was enhanced after treatment with PGE 2, with the greatest effect seen at 60 mins for all 3 PGE 2doses. This response was dose dependent with a 290%, 550% & 1,000% increase in Myeov expression for 0.00025 μ M, 0.1 μ M and 1 μ M PGE 2respectively. Conclusion: In addition to promoting CRC proliferation and invasion, our findings indicate that Myeov stimulates CRC cell migration, and its expression may be PGE 2dependant. © 2010 Lawlor et al; licensee BioMed Central Ltd.