Abstract
The fluorescent stain Nile Red has been used extensively for the quantification of lipids in phytoplankton, including microalgae, because it preferentially stains neutral lipids and it is economical and sensitive to use for screening purposes. Although its basic application has not changed for several decades, recent improvements have been made to improve its utility across applications. Here we describe additional refinements in its application and interpretation as a high-throughput method for the rapid quantification of neutral lipids in liquid cultures of marine phytoplankton. Specifically we address (1) interspecies comparisons, (2) fluorescence excitation and emission wavelengths, and (3) the time course of the Nile Red signal in the context of using bulk or cell-specific fluorescence to
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CITATION STYLE
Johnson, Z. I., Bidigare, R. R., Blinebry, S. K., Brown, S. L., Cullen, J. J., Loftus, S. E., … Van Mooy, B. A. S. (2017). Screening for Lipids From Marine Microalgae Using Nile Red. In Consequences of Microbial Interactions with Hydrocarbons, Oils, and Lipids: Production of Fuels and Chemicals (pp. 1–22). Springer International Publishing. https://doi.org/10.1007/978-3-319-31421-1_382-1
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