Recent studies have demonstrated the importance of regulatory B cells in autoimmune, allergic, and inflammatory diseases. These B cells have an ability to suppress excessive immune reactions by multiple mechanisms. Most studies have focused on IL-10-producing B cells, but we have previously reported that transforming growth factor (TGF)-β1 secretion by B cells also plays an important role in intestinal homeostasis and mucosal inflammation. B cell-secreted TGF-β may be involved in the regulation of T cell immunity (differentiation, proliferation, and apoptosis) that is relevant to the pathogenesis of autoimmune or inflammatory disease. Here, we provide detailed instruction for detecting and measuring TGF-β produced by B cells. © 2014 Springer Science+Business Media New York.
CITATION STYLE
Mishima, Y., Ishihara, S., Hansen, J. J., & Kinoshita, Y. (2014). TGF-β detection and measurement in murine B cells: Pros and cons of the different techniques. Methods in Molecular Biology, 1190, 71–80. https://doi.org/10.1007/978-1-4939-1161-5_6
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