Novel assessment of urinary albumin excretion in type 2 diabetes patients by Raman spectroscopy

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Abstract

Urinary albumin excretion remains the key biomarker to detect renal complications in type 2 diabetes. As diabetes epidemy increases, particularly in low-income countries, efficient and low-cost methods to measure urinary albumin are needed. In this pilot study, we evaluated the performance of Raman spectroscopy in the assessment of urinary albumin in patients with type 2 diabetes. The spectral Raman analysis of albumin was performed using artificial urine, at five concentrations of albumin and 24 h collection urine samples from ten patients with Type 2 Diabetes. The spectra were obtained after removing the background fluorescence and fitting Gaussian curves to spectral regions containing features of such metabolites. In the samples from patients with type 2 diabetes, we identified the presence of albumin in the peaks of the spectrum located at 663.07, 993.43, 1021.43, 1235.28, 1429.91 and 1633.91 cm−1. In artificial urine, there was an increase in the intensity of the Raman signal at 1450 cm−1, which corresponds to the increment of the concentrations of albumin. The highest concentration of albumin was located at 1630 cm−1. The capability of Raman spectroscopy for detection of small concentrations of urinary albumin suggests the feasibility of this method for the screening of type 2 diabetes renal complications.

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APA

Flores-Guerrero, J. L., Muñoz-Morales, A., Narea-Jimenez, F., Perez-Fuentes, R., Torres-Rasgado, E., Ruiz-Vivanco, G., … Castro-Ramos, J. (2020). Novel assessment of urinary albumin excretion in type 2 diabetes patients by Raman spectroscopy. Diagnostics, 10(3). https://doi.org/10.3390/diagnostics10030141

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