Abstract
A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and co-culture of oocyte-cumulus complexes is reported. Sodium alginate forms strong gels when jellified with barium ions, allowing the self-organization of cells into a 3D structure. This method of encapsulation is simple and cheap, allowing the culture of cells in a 3-dimensional fashion.
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APA
Faustini, M., Curone, G., Torre, M. L., & Vigo, D. (2018). Bioencapsulation of Oocytes and Granulosa Cells. In Methods in Molecular Biology (Vol. 1817, pp. 89–93). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8600-2_9
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