Development of Soft Rice Lines by Regulating Amylose Content via Editing the 5′UTR of the Wx Gene

6Citations
Citations of this article
9Readers
Mendeley users who have this article in their library.

Abstract

The type of soft rice with low amylose content (AC) is more and more favored by consumers for its better eating and cooking quality, as people’s quality of life continuously improves in China. The Wx gene regulates the AC of rice grains, thus affecting the degree of softness of the rice. Mei Meng B (MMB), Tian Kang B (TKB), and DR462 are three indica rice maintained lines with good morphological characters, but also with undesirably high AC. Therefore, CRISPR/Cas9 technology was used to edit the Wx gene of these lines to create a batch of soft rice breeding materials. New gene-edited lines MMB-10-2, TKB-21-12, and DR462-9-9, derived from the above parental lines, respectively, were selected in the T2 generations, with an AC of 17.2%, 16.8%, and 17.8%, and gel consistency (GC) of 78.6 mm, 77.4 mm, and 79.6 mm, respectively. The rapid viscosity analysis (RVA) spectrum showed that the three edited lines had a better eating quality as compared to the corresponding wild type, and showing new characteristics, different from the high-quality soft rice popular in the market. There was no significant difference in the main agronomic traits in the three edited lines compared to the corresponding wild types. Moreover, the chalkiness of DR462-9-9 was reduced, resulting in an improved appearance of its polished rice. The present study created soft rice germplasms for breeding improved quality hybrid rice, without changing the excellent traits of their corresponding wild type varieties.

References Powered by Scopus

Rapid isolation of high molecular weight plant DNA

10378Citations
N/AReaders
Get full text

A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase

1830Citations
N/AReaders
Get full text

A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants

1759Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Molecular and genetic bases of rice cooking and eating quality: An updated review

8Citations
N/AReaders
Get full text

Fine mapping of the grain chalkiness quantitative trait locus qCGP6 reveals the involvement of Wx in grain chalkiness formation

5Citations
N/AReaders
Get full text

Variations and Trends in Rice Quality across Different Types of Approved Varieties in China, 1978–2022

3Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Yang, J., Guo, X., Wang, X., Fang, Y., Liu, F., Qin, B., & Li, R. (2022). Development of Soft Rice Lines by Regulating Amylose Content via Editing the 5′UTR of the Wx Gene. International Journal of Molecular Sciences, 23(18). https://doi.org/10.3390/ijms231810517

Readers' Seniority

Tooltip

Researcher 1

100%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 2

67%

Engineering 1

33%

Article Metrics

Tooltip
Mentions
Blog Mentions: 1

Save time finding and organizing research with Mendeley

Sign up for free