Multiplexed assay chemistries provide for multiple measurements of cellular parameters within a single assay well. This experimental practice is not only more cost efficient, but also provides more information about a compound or treatment. The ability to combine the activity profiles within the same sample provides a level of normalization not possible with parallel assays. Furthermore, multiplexing caspase activity assays with viability and/or cytotoxicity assays can support conclusions regarding cytotoxic mechanism and provide normalization, which may help correct for differences in cell number.
CITATION STYLE
Niles, A. L., & Riss, T. L. (2015). “Multiplexed viability, cytotoxicity, and caspase activity assays.” Methods in Molecular Biology, 1219, 21–33. https://doi.org/10.1007/978-1-4939-1661-0_3
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