Effect of salinity on polyamines and ethylene in Atriplex prostrata and Plantago coronopus

Abstract

The aim of this study was to investigate the effects of salinity on germination, seedling growth, free polyamines (putrescine, spermidine, and spermine), and ethylene metabolism of two species (Atriplex prostrata Bouchér and Plantago coronopus L.) with different salt sensitivities. Seeds collected from Barranco Hondo (salt marshes, Jaén, southern Spain) were germinated at 0, 50, 100, and 200 mM NaCl in a growth chamber. The germination of P. coronopus seeds decreased considerably with an increasing NaCl concentration, however, seeds of A. prostrata showed high germination percentages (84, 87, and 80 %) at 0 (control), 50, and 100 mM NaCl, respectively, and only at 200 mM NaCl, the germination was reduced to 25 %. In the early phase of vegetative growth (8-d-old seedlings), the fresh mass increased in A. prostrata at 50 and 100 mM NaCl but the fresh mass of P. coronopus showed no significant differences. With respect to polyamines, there was a decrease of the putrescine and spermidine content at all the NaCl treatments, however, the spermine content increased and was much higher in P. coronopus than in A. prostrata. The ethylene, 1-aminocyclopropane-1-carboxylic acid content, and the 1-amino-cyclopropane-1-carboxylic acid synthase activity increased with the increasing NaCl concentration in A. prostrata, and only the ethylene content in P. coronopus. These results indicate that P. coronopus increased the free spermine content, whereas A. prostrata increased the ethylene biosynthetic pathway in order to survive in the saline conditions.