Bloodstream bacterial infections are known to illicit a systemic immune response that can lead to multiorgan failure and septic shock. The current endotoxin identification techniques in serum are expensive and elaborate requiring bulky benchtop instrumentation. We demonstrate a new route for endotoxin detection in which lipopolysaccharides (LPS) in solution are entrapped using C-18 silane-functionalized glass slides and tagged with polymyxin B sulfate (PMB) drug-conjugated gold nanoparticles. The signal from the particles is further amplified via the silver reduction approach to yield concentration-dependent colorimetric spots visible to the bare eye. The method is rapid, reliable, and cost-effective and fulfills an urgent unmet need in the healthcare industry for early septicemia diagnosis.
CITATION STYLE
Kalita, P., Dasgupta, A., & Gupta, S. (2017). Endotoxin esntrapment on glass via C-18 self-assembled monolayers and rapid detection using drug-nanoparticle bioconjugate probes. In Methods in Molecular Biology (Vol. 1600, pp. 133–142). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6958-6_13
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