Molecular etiology of a dominant form of type III hyperlipoproteinemia caused by R142C substitution in apoE4

Abstract

We have used adenovirus-mediated gene transfer in apolipoprotein (apo)E -/- mice to elucidate the molecular etiology of a dominant form of type III hyperlipoproteinemia (HLP) caused by the R142C substitution in apoE4. It was found that low doses of adenovirus expressing apoE4 cleared cholesterol, whereas comparable doses of apoE4[R142C] greatly increased plasma cholesterol, triglyceride, and apoE levels, caused accumulation of apoE in VLDL/IDL/LDL region, and promoted the formation of discoidal HDL. Co-expression of apoE4[R142C] with lecithin cholesterol acyltransferase (LCAT) or lipoprotein lipase (LPL) in apoE -/- mice partially corrected the apoE4[R142C]- induced dyslipidemia. High doses of C-terminally truncated apoE4[R142C]-202 partially cleared cholesterol in apoE -/- mice and promoted formation of discoidal HDL. The fi ndings establish that apoE4[R142C] causes accumulation of apoE in VLDL/IDL/LDL region and affects in vivo the activity of LCAT and LPL, the maturation of HDL, and the clearance of triglyceride-rich lipoproteins. The prevention of apoE4[R142C]-induced dyslipidemia by deletion of the 203-299 residues suggests that, in the full-length protein, the R142C substitution may have altered the conformation of apoE bound to VLDL/IDL/LDL in ways that prevent triglyceride hydrolysis, cholesterol esterifi cation, and receptor-mediated clearance in vivo. -Vezeridis, A. M., K. Drosatos, and V. I. Zannis. Molecular etiology of a dominant form of type III hyperlipoproteinemia caused by R142C substitution in apoE4. J. Lipid Res. 2011. 52: 45-56. © 2011 by the American Society for Biochemistry and Molecular Biology, Inc.