Tailored protection against plasmalemmal injury by annexins with different Ca2+ sensitivities

Abstract

The annexins, a family of Ca2+- and lipid-binding proteins, are involved in a range of intracellular processes. Recent findings have implicated annexin A1 in the resealing of plasmalemmal injuries. Here, we demonstrate that another member of the annexin protein family, annexin A6, is also involved in the repair of plasmalemmal lesions induced by a bacterial pore-forming toxin, streptolysin O. An injury-induced elevation in the intracellular concentration of Ca2+ ([Ca2+]i) triggers plasmalemmal repair. The highly Ca2+-sensitive annexin A6 responds faster than annexin A1 to [Ca2+]i elevation. Correspondingly, a limited plasmalemmal injury can be promptly countered by annexin A6 even without the participation of annexin A1. However, its high Ca2+ sensitivity makes annexin A6 highly amenable to an unproductive binding to the uninjured plasmalemma; during an extensive injury accompanied by a massive elevation in [Ca2+]i, its active pool is severely depleted. In contrast, annexin A1 with a much lower Ca2+ sensitivity is ineffective at the early stages of injury; however, it remains available for the repair even at high [Ca2+]i. Our findings highlight the role of the annexins in the process of plasmalemmal repair; a number of annexins with different Ca2+-sensitivities provide a cell with the means to react promptly to a limited injury in its early stages and, at the same time, to with stand a sustained injury accompanied by the continuous formation of plasmalemmal lesions. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.