Prediction of M2 Macro and Micro-Mutation Frequency Based on M1 Effect in Greengram [Vigna radiata (L.) Wilczek]

Abstract

Mutation breeding requires handling of large population as chances of induction and detection of mutation in a particular gene is rare. This increases the cost of breeding and makes the selection procedure time consuming and tedious. Detection of effective mutagenic treated population in early generations, particularly in M1 generation is no doubt reduce the population load in subsequent generation and thus cost of breeding and provide better scope of selection. Hence, the present investigation was undertaken in greengram [Vigna radiata (L.) Wilczek] using two varieties and the widely used physical mutagen gamma ray at five different doses to find out the possibility of existence of any relationship between M1 estimates and M2 mutation frequency. Results of the study indicated positive relationship between M1 injury and lethality with M2 macro- and micro-mutation frequency which may help in early prediction of mutation frequency.