Inhibition of a CI- - Transporting P-type ATpase in Aplysia gut

Abstract

Utilizing a basolateral membrane vesicle preparation containing Cl- -ATPase from Aplysia foregut, it was shown that orthovanodate inhibited Cl--ATPase activity, ATP-dependent Cl- transport and ATP-dependent membrane potential change. N-ethylmalemide (NEM) and p-chloromercurobenzoate (PCMBS) also inhibited the Cl- pump biochemical and transport characteristics. However, bafilomycin, azide, DCCD or efrapeptin had no effect on the CP pump characteristics suggesting that this Cl- pump was a P-type ATPase.