Transcriptional analysis of the three phosphoglycolate phosphatase genes in wild type and the pgp1 mutant of chlamydomonas reinhardtii

Abstract

Phosphoglycolate phosphatase (PGPase) is an essential enzyme in the photorespiration pathway in plants and photosynthetic algae. In Chlamydomonas reinhardtii, mutants deficient in phosphoglycolate phosphatase (PGPase-1) were reported to require elevated levels of CO2 for growth in the light. Here we described that thispgp1 mutant reverted naturally and regained the ability to survive under low CO2 conditions. Quantitative RT-PCR analysis demonstrated that the upregulation of PGP2 in the pgp1 revertants might contribute to this reversion in the growth phenotype.