One-pot dual-labeling of a protein by two chemoselective reactions

Abstract

Dual system for proteins: The site-specific two-color labeling of a Rab GTPase was achieved in a one-pot procedure by combination of chemoselective native chemical ligation and oxime ligation. This strategy could be a general, facile, and efficient method for specific multiple modifications of a given protein. The Rab GTPase biosensor was demonstrated for protein folding and protein-protein interaction studies. © 2011 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.