Procedure to develop binodal curve and phase diagram for aqueous two-phase system

Abstract

Purification of protein is normally involved an expensive downstream processing step. For large scale manufacturing facilities, identification of an economical procedure is the most advantage. Aqueous Two-Phase System (ATPS) is a technique that suitable to be used in extracting protein from the mix culture supernatant. The process is also economical. However, there are a few tricky parts that the researchers need to master before this technique can be applied. Therefore, this chapter presents the step by step procedure in optimizing this technique.