Studying MHC class II transport in dendritic cells

Abstract

Professional antigen presenting cells, such as dendritic cells, are effective in activating T lymphocytes due to their unique ability to present antigens in the context of both MHC class I and II molecules. After successful loading with antigenic peptides MHC class II molecules traf fi c from the late endosomal loading compartment to the plasma membrane to exert their function of presenting peptides to T helper lymphocytes. Various processes play a role in this event, which are only partly understood to date. The following protocols demonstrate a strategy of how to integrate high throughput datasets to select candidates possibly involved in MHC class II transport for in depth studies. A combination of proteomics, RNAinterference and biochemical experimentation can uncover novel pathways regulating transport processes in primary dendritic cells. © Springer Science+Business Media, LLC 2013.