Analysis of Genotype Composition of Citrus tristeza virus Populations Using Illumina Miseq Technology

Abstract

Recent research describing the strain-specific mechanisms underlying experimental CTV superinfection exclusion has far-reaching implications for the manner in which cross-protecting sources should be selected for. The strain composition of both cross-protecting sources and field populations needs to be sufficiently characterized to improve control of severe stem-pitting and decline isolates. Many of the biological, serological, and molecular techniques used in previous studies yield very limited information about the strain composition of populations and the relative titer of their components. In this chapter we describe a protocol for the characterization of CTV populations, based on the use of the next-generation sequencing Illumina MiSeq platform of p33 gene amplicons.