Sudden death in a rat subarachnoid hemorrhage model

Abstract

The pathogenesis of sudden death during subarachnoid hemorrhage (sah) still remains to be elucidated. A new rat common carotid artery-prechiasmal extracorporeal shunt model was designed to study the effect of different severities of Sah on intracranial pressure (ICP), regional cerebral blood flow (rCBF), and mortality. Different severities of Sah were induced by controlling the bleeding period (from 30 to 90 sec) and number of bleedings (one or three times). Sah caused a dramatic increase in Icp and immediate depression of rcbf, which recovered slowly to a certain extent. Icp increased sharply within the first 30 seconds and reached a plateau concomitant with nearly zero rcbf, which suggested the occurrence of cerebral circulation arrest. Bleeding of more than 60 seconds and increased Icp over 80 mmhg were directly correlated with the mortality. Respiratory arrest was the first sign of death, immediately followed by cardiac depression resulting in sudden death. This model combines arterial bleeding with systemic blood pressure and controlled bleeding time to simulate the acute period of Sah.