Background: A simple and sensitive CE method was developed and validated for the analysis of some beta blockers in human urine. Methods: In this study, salting-out assisted LLE combined with field-amplified sample stacking method was employed for biological sample clean-up and sensitivity enhancement in CE. Results: Under the optimal conditions good linearity (r2 ≥0.998) was obtained, within 0.025-1 μg/ml for propranolol and metoprolol, and within 0.05-1 μg/ml for carvedilol in urine samples. LODs and LLOQs ranged from 0.005 to 0.015 μg/ml, and from 0.025 to 0.05 μg/ml, respectively. The RSDs of intra- and inter-day analysis of examined compounds were less than 4.0%. The recoveries were in the range of 98-119%. Conclusion: The validated method is successfully applied to determine propranolol, metoprolol and carvedilol in human urine samples obtained from the patients who received these drugs. © 2014 Future Science Ltd.
CITATION STYLE
Fazeli-Bakhtiyari, R., Sorouraddin, M. H., Farajzadeh, M. A., Somi, M. H., & Jouyban, A. (2014). Salt-assisted LLE combined with field-amplified sample stacking in CE for improved determination of beta blocker drugs in human urine. Bioanalysis, 6(3), 319–334. https://doi.org/10.4155/bio.13.303
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