Bacterioplankton turnover of dissolved free monosaccharides in a mesotrophic lake

Abstract

Concentrations and bacterioplankton uptake of dissolved free monosaccharides (DFCHO) in relation to bacterioplankton production and phytoplankton biomass were studied in mesotrophic Lake Constance, Germany, from August 1995 to December 1996. Concentrations of total DFCHO, measured by high-performance liquid chromatography (HPLC) and pulsed amperometric detection, ranged from <20 to 440 nM and exhibited pronounced spatiotemporal variations. Enhanced concentrations of DFCHO were often associated with the breakdown of phytoplankton blooms and periods of low phytoplankton growth. Bacterial uptake rates of DFCHO were also enhanced during such events, as well as at peaks of bacterioplankton production (BP). Ratios of uptake of DFCHO/BP, however, indicated that DFCHO concentrations were relatively more important as substrates during periods of low bacterial growth than during phases of intense bacterial growth. Ratios of bacterial uptake of DFCHO/BP from spring to fall ranged from 0.22 to 0.39 in the layer 0-10 m, from 0.17 to 0.56 at 20 m, and from 0.22 to 0.46 at 50 m, respectively. Glucose, galactose, and mannose dominated the DFCHO pool and constituted ~55 to ~70 mol%. The analysis of individual turnover times of five DFCHO concentrations from August to November 1995 at 3, 10, and 50 m revealed that the glucose pool turned over most rapidly, followed by galactose and mannose. Glucosamine and fructose always exhibited substantially longer turnover times. Comparisons to previous studies show that DFCHO concentrations are of similar significance for bacterioplankton growth as dissolved free amino acids (DFAA), even though temporal uptake patterns of both substrates are different.