Development of novel composite antioxidant multiple lipid particles from combination of W/O/W multiple emulsions and solid lipid nanoparticles

Abstract

A novel drug delivery system, multiple solid particles (MLPs), combining the advantages of water-in-oil-in-water (W/O/W) multiple emulsions and solid lipid nanoparticles (SLN) was proposed. The MLPs were produced by simply adding the solid lipid in liquid lipid of W/O/W multiple emulsions and removing the outer aqueous phase of W/O/W multiple emulsions. MLPs could form typical W/O/W multiple emulsion microstructure by self-emulsifying after dispersing in water. MLPs were used to encapsulate both coenzyme Q10 and tea polyphenols (CT-MLPs), and encapsulation efficiency measurement revealed that both coenzyme Q10 and tea polyphenols had high encapsulation efficiency (98.14% and 99.36%, respectively). The effect of the factors on viscosity, stability, and solubility of CT-MLPs were investigated, and the results showed that increasing the concentration of solid lipid glycerin monostearate or hydrophilic emulsifier polyglyceryl-10 laurate, or decreasing the concentration of the inner aqueous phase could increase the viscosity of CT-MLPs. Consequently, the stability of CT-MLPs was improved by increasing the viscosity of the oil phase. Moreover, addition of glycerin monostearate also increased the water solubility of CT-MLPs. During a 60-day stability study, CT-MLPs showed terrific stability and high retention ratio (above 83.82%) at various conditions.