Plasma-Activated Aerosolized Hydrogen Peroxide (aHP) in Surface Inactivation Procedures

Abstract

Introduction: Hydrogen peroxide is a strong oxidant that possesses an antimicrobial activity. It has been successfully used in surface/room decontamination processes either under the form of hydrogen peroxide vapor (HPV) or of vaporized hydrogen peroxide (VHP). Aerosolized hydrogen peroxide (aHP) offers a third alternative. The technology relies on the dispersion of aerosols of a hydrogen peroxide solution often complemented with silver cations. aHP provides an inexpensive and safe approach to treat contaminated rooms but sometimes fails to achieve the 6-log10 reduction limit in the number of viable microorganisms. Methods: Here, we used a venturi-based aHP generator that generates 4 mm in size aerosols from a 12% plasma-activated hydrogen peroxide solution free of silver cations. Results & Discussion: We could successfully and constantly inactivate bacterial growth from biological indicators containing at least 10 6 spores of Geobacillus stearothermophilus placed on stainless steel discs wrapped in Tyvek pouches. We could also show that the biological indicators placed at various locations in a class II biosafety cabinet were equally inactivated, showing that hydrogen peroxide aerosols migrate through HEPA filters. Conclusions: Considering that our method for aerosol generation is simple, reproducible, and highly effective at inactivating spores, our approach is expected to serve as a relatively cost effective alternative method for disinfecting potentially contaminated rooms or surfaces.