A Dystrophin‐Immunoreactive Protein in Mammalian Brain

Abstract

Abstract: Dystrophin is expressed only in muscle and brain, but is absent from all tissues of the adult mdx mouse, a mutant with a single base substitution in the dystrophin gene. The brains of both normal and mdx mice contain a protein of ∼230 kDa that is recognised by anti‐dystrophin antibodies raised to the N‐terminal region of the rod‐like domain. Although the N‐terminal and central rod regions of dystrophin share structural homologies with spectrin, the 230‐kDa protein represents neither of the presently described forms of brain spectrin by a variety of criteria (molecular weight, cerebellar localisation, and developmental regulation) and is distinct from the product of the dystrophin gene. Studies of mdx and normal mouse brain show different postnatal developmental regulation of the 230‐kDa dystrophin‐immunoreactive protein. Copyright © 1993, Wiley Blackwell. All rights reserved