Affinity enrichment techniques in combination with quantitative proteomics enable the unbiased identification of protein-protein interaction, and thus the delineation of protein complexes and interaction networks. Here, we describe an immunoaffinity enrichment approach that employs covalently immobilized antibodies for the identification of protein-protein interactions of endogenously expressed proteins under near-to-physiological conditions. Specifically enriched proteins are identified using shotgun mass spectrometry and isobaric mass tag-based relative quantification.
CITATION STYLE
Dittmann, A., Ghidelli-Disse, S., Hopf, C., & Bantscheff, M. (2014). Mapping protein complexes using covalently linked antibodies and isobaric mass tags. Methods in Molecular Biology, 1156, 279–291. https://doi.org/10.1007/978-1-4939-0685-7_19
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