Proteolytic degradation of von Willebrand factor after DDAVP administration in normal individuals

33Citations
Citations of this article
27Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

The infusion of 1-deamino-8-D-arginine vasopressin (DDAVP) in normal individuals is followed by an increase in factor VIII/von Willebrand factor (vWF) in plasma, by an increase in intensity of all sizes of multimers, and by the appearance of larger multimers of vWF than those seen in the resting state. Since the larger multimers are rapidly cleared and proteolysis is known to cause disaggregation of large multimers, we evaluated the degree of vWF proteolysis after DDAVP administration. DDAVP was infused into eight normal adult volunteers, and the relative proportions of the intact 225 kilodalton (kDa) subunit and the 189, 176, and 140 kDa vWF fragments were compared before and at different times after DDAVP infusion. The relative proportion of the 176 kDa fragment was increased, whereas that of the other species was decreased, thereby indicating that proteolytic fragmentation had occurred. However, plasmin did not appear to be responsible because the vWF fragments characteristically produced by this enzyme could not be detected. Concomitant analysis of vWF multimeric structure showed that these changes were accompanied by an increase in the relative proportion of the satellite bands, which suggests that they were proteolytically generated. Proteolysis may explain, at least in part, rapid clearance of larger vWF multimers released by DDAVP.

Cite

CITATION STYLE

APA

Batlle, J., Lopez-Fernandez, M. F., Lopez-Borrasca, A., Lopez-Berges, C., Dent, J. A., Berkowitz, S. D., … Zimmerman, T. S. (1987). Proteolytic degradation of von Willebrand factor after DDAVP administration in normal individuals. Blood, 70(1), 173–176. https://doi.org/10.1182/blood.v70.1.173.173

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free