Development and characterization of est-ssr markers for tricyrtis sect. Tricyrtis (liliaceae)

Abstract

A set of 8 polymorphic EST-SSR markers has been developed to evaluate the genetic diversity and genetic structure of the populations of Tricyrtis sect. Tricyrtis (Liliaceae) in Kyushu, which reproduces sexually and asexually by bulbils. The total number of alleles for each locus ranged from 2 to 11 with an average of 5.5, while the values of observed and expected heterozygosity ranged from 0.043 to 0.875 and 0.043 to 0.849, respectively. The combined probabilities of identity (PI = 6.0E-07 and PI-Sib = 2.7E-03) of these markers suggest that they are useful for not only evaluating genetic diversity but also for estimating clonal diversity within populations. Cross-species amplification was evaluated for three phylogenet-ically related species, T. macropoda, T. setouchiensis, and T. affinis, to show that all the EST-SSR markers are transferrable among these species.