Somatic embryogenesis in long-term cultures of Gentiana Lutea L. in the presence of osmotic stress

Abstract

Somatic embryogenesis (SE) represents the most efficient way to regenerate plants, providing material for preservation and basic research. Biotechnological approaches are suitable to produce and to preserve plant material in reduced spaces with the protection against biotic and abiotic factors. Recurrent SE was developed in long-term cultures of Gentiana lutea, in the presence of moderate osmotic stress induced by sugar alcohols, without any growth factor. The efficiency of SE in long-term cultures in the presence of mannitol or sorbitol produced better results compared to those in the presence of plant growth regulators. SE can occur as a continuous process, and embryos can be maturated and converted on the same culture medium. The absence of plant growth regulators has a beneficial effect on the quality of the regenerants.