We present here a general methodology for significantly increasing the number of dye/drug molecules that can be attached per protein molecule. As a demonstration of this approach, poly(acrylic acid) (PAA)-based near-infrared fluorescence (NIRF) dye- and glucose-incorporated novel copolymers were synthesized, which were further employed for bioconjugation to avidin and bovine serum albumin (BSA). In this method, azide-terminated poly(tert-butyl acrylate) was synthesized via atom transfer radical polymerization (ATRP). Subsequent deprotection was performed to yield poly(acrylic acid) (PAA) possessing a reactive chain-end. A one-pot sequential amidation of the PAA with the amine derivatives of a nearinfrared fluorescent dye (ADS832WS) and glucose produced NIRF dye-incorporated water-soluble copolymers. End-group modifications were performed to produce alkyne/biotin-terminated copolymers, which were further employed to generate dye-incorporated polymer-protein hybrids via the biotin-avidin interaction with avidin or by “click” bioconjugation with azide-modified BSA.
CITATION STYLE
Dolai, S., Shi, W., Mondal, B., & Raja, K. (2011). Synthesis of drug/dye-incorporated polymer-protein hybrids. In Methods in Molecular Biology (Vol. 751, pp. 29–42). Humana Press Inc. https://doi.org/10.1007/978-1-61779-151-2_3
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