IL-1 induces rapid phosphorylation of the IL-1 receptor.

  • Gallis B
  • Prickett K
  • Jackson J
  • et al.
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Abstract

The IL-1R on murine T cells is a Mr = 80,000 plasma membrane glycoprotein. cDNA cloning and transfection experiments have shown that this is an integral membrane protein, which binds both IL-1 alpha and IL-1 beta and transduces the IL-1 signal. A mAb, RM-5, which binds an epitope on the receptor which is distinct from the IL-1 binding site has been produced in rats. RM-5 has been used to immunoprecipitate the IL-1R from 32P-orthophosphate labeled CHO cells which express approximately 100,000 functional, murine rIL-1R/cell. Phosphorylation of the receptor was observed as early as 1 min after the addition of IL-1 and continued for periods of up to 30 min. Phosphorylation increases as the concentration of IL-1 increases from 10(-13) to 10(-8) M. Potassium hydroxide hydrolysis of the phosphorylated IL-1R shows that more than 90% of the phosphate is incorporated into serine or threonine. Thus, one of the earliest events after IL-1 binding to the IL-1R is activation of a serine/threonine protein kinase and phosphorylation of the IL-1R itself.

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APA

Gallis, B., Prickett, K. S., Jackson, J., Slack, J., Schooley, K., Sims, J. E., & Dower, S. K. (1989). IL-1 induces rapid phosphorylation of the IL-1 receptor. The Journal of Immunology, 143(10), 3235–3240. https://doi.org/10.4049/jimmunol.143.10.3235

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