Sequencing of cDNA clones from the genetic map of tomato (Lycopersicon esculentum)

Abstract

The dense RFLP linkage map of tomato (Lycopersicon esculentum) contains > 300 anonymous cDNA clones. Of those clones, 272 were partially or completely sequenced. The sequences were compared at the DNA and protein level to known genes in databases. For 57% of the clones, a significant match to previously described genes was found. The information will permit the conversion of those markers to STS markers and allow their use in PCR-based mapping experiments. Furthermore, it will facilitate the comparative mapping of genes across distantly related plant species by direct comparison of DNA sequences and map positions.