Quantitative analysis of conjunctival microvasculature imaged using optical coherence tomography angiography

Abstract

Background: The goal was to quantitatively analyze the bulbar conjunctival microvascular density using optical coherence tomography angiography (OCTA) and compare it to the vessel density using functional slit-lamp biomicroscopy (FSLB). Methods: Temporal bulbar conjunctiva of 20 eyes (10 healthy subjects) was imaged using both OCTA and FSLB. Image processing was performed including equalization, de-noising, thresholding, and skeletonization. The vessel density was measured by fractal analysis (box counting, Dbox) and pixel counting (%). Results: Vessel density (Dbox) of the bulbar conjunctiva obtained using OCTA was 1.28 ± 0.01 Dbox, which was significantly lower than the result (1.32 ± 0.01 Dbox, P < 0.001) obtained using FSLB. Furthermore, the vessel density (%) obtained using OCTA was 3.31 ± 0.12%, which was also significantly lower than the result (3.69 ± 0.16%, P < 0.001) obtained using FSLB. No significant correlations (r ranged from 0.21 to 0.32, P > 0.05) between both instruments were found in both vessel density methods (Dbox and percentage). However, in each of the devices, vessel density in Dbox was significantly correlated with the vessel density in percentage (r = 1.0 for FSLB and r = 0.98 for OCTA, both P < 0.001). Conclusion: This study demonstrated that the vessel density of the bulbar conjunctiva obtained using OCTA can be quantified, and the results were not compatible with that obtained using slit-lamp biomicroscopy photography.